Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens

Most biosurfactants are obtained using costly culture media, which limits their wider industrial use. In the present study, a low-cost medium, containing agro-industrial residue black cumin cake, was developed for amphisin production by Pseudomonas fluorescens DSS73. By cake as substrate, not only cost reduced but also higher yield achieved. A Box-Behnken experimental design applied to maximize lipopeptide biosurfactant production. The optimal conditions production, such (6.6%) and NaCl (8.0 mM) concentration, cultivation time (6.5 days), were determined. Yield of performed in conditions, reached 16.51 ± 0.49 g/L. Such high has been evidenced previously biosurfactants. Moreover, active utilization observed with aid scanning electron microscopy (SEM), documented numerous holes pitting on surface, confirmed. Finally, antifungal activity against Aspergillus carbonarius demonstrated. Hence, P. achieved statistical optimization an inexpensive by-product first time.